Summary

The Bround Lab studies mitochondrial biology with an emphasis on heart and muscle physiology and disease. Mitochondria are important organelles which serve as the powerhouse and molecular forge of the cell, generating energy and building many key components that make up cells. The lab studies how cells communicate with mitochondria to coordinate energy production and maintain mitochondrial quality control, but also how mitochondria participate in cell death and disease. The Bround lab utilizes molecular biology, cell biology, and animal physiology experiments to study mitochondrial function from the protein level to the whole organism level. We are interested in learning how to maximize mitochondrial function in health and how to prevent mitochondria-dependent cell death in heart disease, muscular dystrophy, and Alzheimer's disease.

The Bround Lab is looking for a Research Technician to participate in ongoing research programs and assist with core laboratory functions. The lab works on molecular, cellular, and animal models, and a Research Technician would participate in all facets of this research.  Research Technician interested in research experience while considering medschool or graduate school will be a great fit. The required training will be provided for all required duties.

Job Duties

• Conducts mouse colony management, animal husbandry, genotyping,  
• Works on Animal surgery that includes heart and muscle injury models.
• Performs animal-related studies that includes in vivo heart and muscle function studies such as echocardiography and muscle force experiments, tissue perfusions experiments, and tissue collection.
• Performs cell culture that maintaining and generating cell lines, live cell imaging, sample collection, primary cell isolation from heart and skeletal muscle. 
• Performs molecular biology, mitochondrial isolation, western blot experiments, qPCR experiments, biochemical assays.
• Conducts tissue sectioning, staining, microscopy, histological data analysis. 
• Orders lab, prepares common reagents and stocks.

Minimum Qualifications

• Associate's degree in a Basic Science or related field. Two years of related experience may substitute for degree requirement.
• One year of relevant experience.

Preferred Qualifications

• Experience with rodent animal husbandry or surgery is a strong plus, but not required.
• Experience with research in lab is desired but not required.