Research Technologist

Johns Hopkins University

Baltimore, MD

ID: 7063408
Posted: February 6, 2020
Application Deadline: Open Until Filled

Job Description

Specific Duties/Responsibilities:

Barcode and enter sample associated information into the database.
Transfer DNA samples into 96-well plate format.
Receive and prepare deepwell DNA stock plates and create working DNA plates.
Determine concentration and volume of DNA samples using various instruments.
Dilute samples to working concentrations.
Maintain and monitor inventory of all in-house DNA samples and reagent supplies.
Create master mixes; Set up polymerase chain reactions; trouble-shoot such reactions.
Operate and troubleshoot robotic liquid handlers.
Prepare and complete SNP genotyping assays including, but not limited to, Illumina assays and workflows.
Prepare samples for targeted capture of specific regions of DNA for sequencing experiments.
This includes library preparation and capture techniques.
Prepare and complete Next-Gen sequencing assays including, but not limited to, Illumina HiSeq workflows.
Perform quality control checks of data using various software, including, but not limited to, GenomeStudio, Spotfire and other in-house QC metrics reports.
Maintain timely and accurate laboratory records (electronic and written).
Collect, organize, interpret and submit experimental data to database and supervisors.
Perform general lab maintenance duties.
Perform validation tests of lab reagents.
Participate in committees that function to improve processes.
Communicate effectively to supervisor and team members.

Minimum Qualifications:

Bachelor’s degree or equivalent in biological sciences or chemistry is required.

Preferred Qualifications:

Some related post-undergraduate experience in a comparable lab or significant research project, not related to normal course requirements.

Special Knowledge, Skills, and Abilities:

Basic understanding of molecular biology and genetics.
Requires the ability to safely operate scientific laboratory equipment.
Machines/equipment used: Centrifuges, Illumina BeadLab system (Tecans, iScans, HiScans, TeFlow), Illumina BeadXpress, Covaris, BioAnalyzer, Nanodrop instruments, Illumina HiSeq, SpriWorks, thermal cyclers, robotic and manual liquid handlers, common laboratory equipment, Macintosh, PC computers, UNIX servers, Oracle database.


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